Over-expression of 72 ku protein of wheat yellow mosaic virus in E-coli and preparation of its antiserum

By reverse transcription-polymerase chain reaction (RT-PCR), cDNA fragment of wheat yellow mosaic virus (WYMV) RNA2 encoding 72 ku protein has been sy nthesized and cloned into plasmid pET30a(+) for overexpression in prokaryot ic cells. BL21(DE3) pLys S of E.coli transformed with the recombinant plasm id pETP72 containing the fragment has been induced to express the 72 ku pro tein on high level. The produced protein has been purified from sodium dode cyl sulfate-polyacrylamide gel (SDS-PAGE) for its antiserum preparation. In Western-blotting analysis, the antibodies reacted with the 72 ku protein e xpressed in E.coli.