Jw. Qiu et al., Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization, CHIN SCI B, 45(6), 2000, pp. 528-532
RAPD and RFLP analyses of double haploid lines which derived from hybridiza
tion between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamag
rass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Opero
n primers have been screened, which stably amplified Tripsacum dactyloides
(male parent) specific bands in the double haploid lines. These results con
firm the fact that Tripsacum dactyloides DNA has been integrated into wheat
genome by sexual hybridization at molecular level. This idea has been furt
her testified by RFLP analysis. Application and potentials of transferring
Tripsacum dactyloides DNA into wheat genome by sexual hybridization in whea
t breeding are discussed.