Establishment of Jurkat tet-on cell line

Tet-control system is developed to tightly control target gene expression i n mammalian cells by using the regulatory elements of tetracycline-represso r of the transposer Tn 10 from E. coli. We have transfected reverse tetracy cline-controlled transactivator gene (rtTA) into genome of Jurkat cells and established two Jurkat tet-on cell lines. Induction of luciferase reporter activity with doxycycline, a tetracycline derivative, is dose-dependent wi th a peak value of 32-fold increment. Establishment of Jurkat tet-on cell l ines greatly facilitates quantitative studies on target gene functions in t he cells.