Cloning of human and mouse GRY-RBP cDNA

After screening human fetal brain cDNA library, a novel human cDNA encoding an RNA-binding protein was cloned and named GRY-RBP. Human GRY-RBP cDNA is 2 932 bp in length, and encodes a protein of 623 amino acids with a deduce d molecular mass of 69.6 ku. Human GRY-RBP contains three RNA recognition m otifs (RRMs) and a glycine (G), tyrosin (Y)-rich C-terminus. Computer searc hes with sequence database revealed its moderate homology with RRMs of many RRM RNA-binding proteins. In this study, the full coding region and partia l non-coding region of mouse GRY-RBP cDNA were amplified by PCR using mouse brain cDNA library as template. Three transcripts of Gry-rbp are expressed in all tissues as shown by Northern blot, but the amounts in heart, plance ta and skeletal muscle are considerably higher than those in other tissues.