Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice

The promoter fragments of wheat GstA1 and potato Gst1 have been amplified b y PCR, cloned and fused respectively to the minimal promoter sequence of ri ce actin gene (Act1)) and its 5' untranslated leader sequence together with GUS. The constructs with 2 chimeric promoters (WGA and PGA) have been tran sferred into rice in order to analyze their inducibility patterns in transg enic rice plants. The results show that: WGA and PGA are both inducible by elicitors of Pyricularia oryzae in transgenic rice cells; the intron I of r ice Act1 gene is important for the heterogenic expression of monocot and di cot promoter elements in rice; and the Act1 minimal promoter and its 5' unt ranslated leader sequence produced low level background expression in rice.