In animal cells, action of acetylcholine depends on its binding with its tw
o specific receptors on the plasma membrane: the nicotinic and muscarinic r
espectively. The present investigation has shown that agonists of muscarini
c receptor (muscarine) could induce stomatal opening, while the antagonists
(atropine) could block stomatal opening induced by acetylcholine. Their ef
fects can only be realized in medium containing Ca2+, but not in medium con
taining K+. The results tend to reveal that the muscarinic receptor is invo
lved in acetylcholine-induced stomatal movement.