Effects of titanium surfaces blasted with TiO2 particles on the initial attachment of cells derived from human mandibular bone - A scanning electron microscopic and histomorphometric analysis

This study was performed to determine the effect of commercially pure titan ium surfaces blasted with TiO2 particles on the biological responses of cel ls derived from human mandibular bone. The morphology and attachment of tho se cells were investigated on turned titanium surfaces (control) and surfac es blasted with 45 mu m (standard), 45-63 mu m, and 63-90 mu m TiO2 particl es. The surfaces were analyzed in a scanning electron microscope. Based on surface analyses reported elsewhere. the turned samples had the smoothest s urfaces and the roughest were those blasted with the largest particles (63- 90 mu m). The cell profile areas were measured using a semi-automatic inter active image analyzer. The attachment was determined as a ratio of the area of cell profiles and the total micrograph area and was expressed as percen tage of attachment. Morphologically, the cells were heterogeneous. In gener al, the cells had spread well on all titanium surfaces, indicating good att achment to both smooth and rough surfaces. After 1, 3 and 6 h, the percenta ge of cell attachment did not differ significantly between the surfaces bla sted with 63-90 um and the turned surfaces, but was significantly lower on the surfaces blasted with 45 mu m or 45-63 mu m particles. After 24 h the s urfaces blasted with 63-90 mu m particles had a higher rate of cell attachm ent than ail the other surfaces including the controls. It is concluded tha t attachment and growth of cells originating from human mandibular bone in vitro, are influenced by the micro-texture of the implant surface.