ACTIVE CONFORMATION OF ALPHA-CHYMOTRYPSIN IN ORGANIC-SOLVENTS AS STUDIED BY CIRCULAR-DICHROISM

Authors
SASAKI T KOBAYASHI M KISE H
Citation
T. Sasaki et al., ACTIVE CONFORMATION OF ALPHA-CHYMOTRYPSIN IN ORGANIC-SOLVENTS AS STUDIED BY CIRCULAR-DICHROISM, Biotechnology techniques, 11(6), 1997, pp. 387-390
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
6
Year of publication
1997
Pages
387 - 390
Database
ISI
SICI code
0951-208X(1997)11:6<387:ACOAIO>2.0.ZU;2-8
Abstract
Circular dichroic (CD) spectra of alpha-chymotrypsin were measured in mixtures of water and ethanol, acetonitrile, or tetrahydrofuran. The c atalytic activity of alpha-chymotrypsin in the different solvents was related to the change of CD bands especially that at 230 nm. The fine structure in near-UV region may also be useful to estimate the catalyt ic activity of alpha-chymotrypsin.