RAPID AND SENSITIVE DETECTION OF BETA-GALACTOSIDASE-PRODUCING YEASTS BY USING MICROTITER PLATE ASSAY

Authors
DOMINGUES L TEIXEIRA JA LIMA N
Citation
L. Domingues et al., RAPID AND SENSITIVE DETECTION OF BETA-GALACTOSIDASE-PRODUCING YEASTS BY USING MICROTITER PLATE ASSAY, Biotechnology techniques, 11(6), 1997, pp. 399-402
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
6
Year of publication
1997
Pages
399 - 402
Database
ISI
SICI code
0951-208X(1997)11:6<399:RASDOB>2.0.ZU;2-C
Abstract
Yeast beta-galactosidase activity was detected by a microtiter plate a ssay using pNPG or Xgal as substrate in 30 minutes. The detection gave a clear result which is well correlated with the specific beta-galact osidase activity present in each strain studied. The microtiter plate assay is an effective method to improve the detection and quantify the beta-galactosidase gene in recombinant strains of Saccharomyces cerev isiae.