RECOVERY OF POLY-3-HYDROXYBUTYRATE FROM RECOMBINANT ESCHERICHIA-COLI BY HOMOGENIZATION AND CENTRIFUGATION

Authors
LING Y WILLIAMS DRG THOMAS CJ MIDDELBERG APJ
Citation
Y. Ling et al., RECOVERY OF POLY-3-HYDROXYBUTYRATE FROM RECOMBINANT ESCHERICHIA-COLI BY HOMOGENIZATION AND CENTRIFUGATION, Biotechnology techniques, 11(6), 1997, pp. 409-412
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
6
Year of publication
1997
Pages
409 - 412
Database
ISI
SICI code
0951-208X(1997)11:6<409:ROPFRE>2.0.ZU;2-I
Abstract
Poly-3-hydroxybutyrate from recombinant E. coli was recovered using ho mogenization and continuous centrifugation with a purity of 94%. Final protein and DNA concentrations were 1.0% w/w and 1.9% w/w, respective ly, when a hypochlorite treatment was employed prior to centrifugation . High fractional cell debris removal (94%) was achieved with two cent rifugation steps.