A COMPARISON OF GEL-FILTRATION CHROMATOGRAPHIC SUPPORTS FOR PLASMID PURIFICATION

Authors
FERREIRA GNM CABRAL JMS PRAZERES DMF
Citation
Gnm. Ferreira et al., A COMPARISON OF GEL-FILTRATION CHROMATOGRAPHIC SUPPORTS FOR PLASMID PURIFICATION, Biotechnology techniques, 11(6), 1997, pp. 417-420
Citations number
4
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
6
Year of publication
1997
Pages
417 - 420
Database
ISI
SICI code
0951-208X(1997)11:6<417:ACOGCS>2.0.ZU;2-4
Abstract
Two gel filtration chromatographic supports, Superose 6 and Sephacryl S1000 SF, were used to purify supercoiled plasmids using a 4.8 kb plas mid as a model. Both supports purified the plasmid from RNA and other small molecular weight contaminants, as shown by agarose electrophores is and ion exchange HPLC, with an overall yield of 70%. Sephacryl S100 0 SF was the better support as it resolved supercoiled, relaxed, linea r and concatamer plasmid forms, and chromosomal DNA.