S. Kurioka et al., Reverse correlation between urine nitric oxide metabolites and insulin resistance in patients with type 2 diabetes mellitus, ENDOCR J, 47(1), 2000, pp. 77-81
We studied the possible relationship between nitric oxide (NO) production a
nd insulin resistance in patients with type 2 diabetes mellitus. Urine NO m
etabolites (NOx) were measured as an index for NO production by HPLC combin
ed with a Cd column, Griess reaction and a spectrophotometer in 403 healthy
control subjects and 102 hospitalized patients with type 2 diabetes. Gluco
se infusion rate (GIR) was measured as a reverse index for insulin resistan
ce by euglycemic glucose clamp study using an artificial pancreas in 20 of
102 diabetic patients. Urine NOx was lower in the patients with type 2 diab
etes than in healthy control subjects (mean+/-SE: 3.18+/-0.02 versus 3.25+/
-0.01 log[mu mol/gCr], p < 0.01). Urine NOx was correlated with body mass i
ndex (BMI) in 102 diabetic patients (r= -0.372, p<0.001), but not related t
o either age, sex, fasting plasma glucose, HbA1(c) or blood pressure. Urine
NOx was correlated with GIR independent of BMI in 20 diabetic patients (r=
0.774, P<0.0001). These findings suggest that NO production is closely rela
ted with insulin resistance in patients with type 2 diabetes.