The amounts of dry microbial biomass in five biogeocenoses of the Okskii Re
serve have been determined. They can be as high as several tens of tons per
hectare. The biomass of eukaryotic organisms significantly exceeds the bio
mass of prokaryotic organisms. Every soil type is characterized by a certai
n pool of microbial biomass and specific patterns of its spatial (in the ve
rtical profile of soil) and temporal (seasonal changes) distribution. Soil
fungi predominate in the microbial biomass and are represented mostly by my
celium and not by spores. The character of seasonal changes in the total po
ol of microbial biomass and in the proportions between different groups of
microorganisms depends on the soil moisture content and soil texture that p
redetermine the water permeability of soils. The number of fungi depends on
season. Forest litters and sod horizons of flood-plain soils are character
ized by the highest values of microbial biomass and species diversity.