Effects of fatty acids, nucleotides and reactive oxygen species on durum wheat mitochondria

Linoleic acid (LA) and other fatty acids added to respiring durum wheat mit ochondria (DWM) were found to cause a remarkable membrane potential (Delta Psi) decrease, as monitored by measuring safranin fluorescence. The rate of Delta Psi decrease showed (i) saturation dependence on LA concentration: ( ii) fatty acid specificity; (iii) inhibition by externally added ATP, GDP, GTP and Mg2+ and (iv) sigmoid dependence upon initial Delta Psi, thus sugge sting the existence of an active plant mitochondrial uncoupling protein (PU MP) in mitochondria from monocotyledonous species (durum wheat, Triticum du rum Desf,), Surprisingly, the rate of the linoleate dependent Delta Psi dec rease was found to be activated by reactive oxygen species (ROS) (hydrogen peroxide and superoxide anion) and, moreover, linoleate proved to lower the mitochondrial generation of superoxide anion, These results suggest that R OS ran activate PUMP, thus protecting the cell against mitochondrial ROS pr oduction, (C) 2000 Federation of European Biochemical Societies.