The rate at which human sperm are immobilized and killed by mild acidity

Objective: To determine the rate at which mild acidity immobilizes and kill s human sperm and to evaluate an acidic microbicide, BufferGel, for sperm i mmobilization. Design: Controlled in vitro study. Setting: An academic research university and hospital andrology lab. Patient(s): Eight volunteer male sperm donors. Intervention(s): Semen samples were treated with hydrochloric acid (HCl) or BufferGel. Main Outcome Measure(s): Sperm motility was measured by using a computerize d automated semen analyzer and video microscopy. Sperm membrane permeabilit y and intracellular pH were measured by using fluorescent techniques. Result(s): In semen acidified with HCl to ptr 4.0, sperm were rapidly immob ilized (within 1 min) and were irreversibly immobilized (killed) within 10 minutes. The speed of immobilization and of killing were both linearly prop ortional to hydrogen ion activity over a pH range of 7.5-4.0. Across the sa me range, the intracellular pH of human sperm equilibrated to within 0.5 pH units of extracellular pH within 1-2 minutes. BufferGel immobilized sperm significantly faster than HCl from pH 4.0-6.0. Conclusion(s): Exposure to mild acidity rapidly acidifies the intracellular pH of human sperm and is rapidly spermicidal. BufferGel accelerates acid i mmobilization of sperm.