Free plasma thyroxine levels in coho salmon, Oncorhynchus kisutch, during parr-smolt transformation comparison with total thyroxine, total triiodothyronine, and growth hormone levels

Free plasma thyroxine (FT4) levels were measured in coho salmon, Oncorhynch us kisutch, during parr-smolt transformation (smoltification) using an equi librium dialysis system followed by a radioimmunoassay. The FT4 data were c orrelated to total plasma thyroxine (TT4), triiodothyronine (TT3), and grow th hormone (GH). Plasma samples were taken weekly from early April to late May, when the salmon where released from the hatchery. Free thyroxine and G H levels increased gradually through smoltification. TT4 levels increased s ignificantly in mid-April and in mid-May. TT3 levels increased in April and remained elevated until late-May after which they declined to the lowest l evels. During the first increase in TT4 levels, FT4 levels remained low and TT3 levels did not increase until FT4 levels increased in late-April. In a ddition, after TT4 levels decreased in late May, FT4 levels remained elevat ed. These data show that there are differences between the plasma FT4 and T T4 profiles during smoltification. Nevertheless, regression analysis indica tes that FT4 levels are highly correlated to the increases in the levels of GH (r=0.73) and TT4 (r=0.70). In addition, GH is less correlated to TT4 an d TT3 (r=0.24 and r=-0.46, respectively) compared with FT4 (r=0.73), sugges ting a close relationship between the increases of FT4 and GH. In addition, these data suggest that this method of measuring free plasma thyroxine may provide a new tool for studying the timing of thyroid hormone action and r egulation during parr-smolt transformation in salmonids.