PCR-RFLP analysis of mitochondrial DNA: Differentiation of fish species

Authors
Wolf, C Burgener, M Hubner, P Luthy, J
Citation
C. Wolf et al., PCR-RFLP analysis of mitochondrial DNA: Differentiation of fish species, FOOD SCIENC, 33(2), 2000, pp. 144-150
Citations number
28
Categorie Soggetti
Food Science/Nutrition
Journal title
FOOD SCIENCE AND TECHNOLOGY-LEBENSMITTEL-WISSENSCHAFT & TECHNOLOGIE
ISSN journal
00236438 → ACNP
Volume
33
Issue
2
Year of publication
2000
Pages
144 - 150
Database
ISI
SICI code
0023-6438(2000)33:2<144:PAOMDD>2.0.ZU;2-7
Abstract
A method for identification of fish species has been developed based on the amplification of a specific part of the mitochondrial genome (tRNA(Glu)/cy tochrome b) using the polymerase chain reaction (PCR). To distinguish betwe en several fish species the obtained 464 bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species specific res triction fragment length polymorphisms (RFLP). The method is suitable for d ifferentiating between, for example, Atlantic and several Pacific salmon sp ecies. (C) 2000 Academic Press.