Structure and localization of mouse PMSCL1 and PMSCL2 genes

Sera from some patients with polymyositis-scleoderma overlap syndrome (PRI- SCL) recognize two antigenically unrelated proteins, PMSCL1 and PMSCL2. Com plete mouse Pmscl1 and Pmscl2 cDNA sequences, chromosomal localizations, ex on/intron structure, and promoter region sequences of the mouse Pmscl2 gene are presented. The PMSCL1 gene was found to overlap significantly with cyc lin A2 in both human and mouse. As such, it may be deduced that PMSCL1 sequ ences map to human chromosome 4q27 and the proximal portion of mouse chromo some (Chr) 3 where human and mouse cyclin A2 genes reside. Analysis of huma n and mouse PMSCL1 cDNA sequences provides evidence that the PMSCL1 protein is 68 amino acids longer than previously thought. A BAC containing mouse P mscl2 was localized to distal mouse Chr 4 by FISH. This BAC contains the mi crosatellite D4Mit310. D4Mit310 colocalizes with a number of genes that map to human 1p36. In fact, a STS (G25404) located 54.6 cR from the top of hum an chromosome 1 was found to contain PMSCL2 sequence upon BLAST search. (C) 2000 Academic Press.