Mm. Nogueras et al., Cloning, sequencing, and role in serum susceptibility of porin II from mesophilic Aeromonas hydrophila, INFEC IMMUN, 68(4), 2000, pp. 1849-1854
We cloned and sequenced the structural gene for Aeromonas hydrophila porin
II from strain AH-3 (serogroup O:34). The genetic position of this gene, li
ke that of ompF in Escherichia coli, is adjacent to aspC and transcribed in
the same direction. However, upstream of the porin II gene no similarities
with E. coli were found. We obtained defined insertion mutants in porin II
gene either in A. hydrophila (O:34) or A. veronii sobria (serogroup O:11)
serum-resistant or -sensitive strains. Furthermore, we complemented these m
utants with a plasmid harboring only the porin II gene, which allowed us to
define the role of porin II as an important surface molecule involved in s
erum susceptibility and C1q binding in these strains.