Antibody-mediated elimination of the obligate intracellular bacterial pathogen Ehrlichia chaffeensis during active infection

It is generally accepted that cellular, but not humoral immunity, plays an important role in host defense against intracellular bacteria. However, stu dies of some of these pathogens have provided evidence that antibodies can provide immunity if present during the initiation of infection. Here,,ve ex amined immunity against infection by Ehrlichia chaffeensis, an obligate int racellular bacterium that causes human monocytic ehrlichiosis. Studies with mice have demonstrated that immunocompetent strains are resistant to persi stent infection but that SCID mice become persistently and fatally infected . Transfer of immune serum or antibodies obtained from immunocompetent C57B L/6 mice to C57BL/6 scid mice provided significant although transient prote ction from infection. Bacterial clearance was observed when administration occurred at the time of inoculation or well after infection was established . The effect was dose dependent, occurred within 2 days, and persisted for as long as 2 weeks. Weekly serum administration prolonged the survival of s usceptible mice. Although cellular immunity is required for complete bacter ial clearance, the data show that antibodies can play a significant role in the elimination of this obligate intracellular bacterium during active inf ection and thus challenge the paradigm that humoral responses are unimporta nt for immunity to such organisms.