Supercritical fluid extraction of grape glycosides

Supercritical fluid extraction with methanol-modified CO2 was used to extra ct glycosides from grapes. An optimization design involving 12 extraction v ariables was applied to achieve quantitative recoveries. The most important factor was the amount of organic modifier, a consequence of the high degre e of glycoside polarity. By the proposed method, the total time of analysis can be decreased relative to that required for more conventional extractio ns. The full method can also be automated.