A. Villanueva et al., Heterologous expression in Aspergillus nidulans of a Trichoderma longibrachiatum endoglucanase of enological relevance, J AGR FOOD, 48(3), 2000, pp. 951-957
An Aspergillus nidulans transformant expressing the Trichoderma longibrachi
atum endoglucanase 1 gene (egl1) has been constructed. The extracellular pr
oduction of EGL1 in different culture media has been studied, and a medium
has been found in which EGL1 is the predominant extracellular protein produ
ced. The enzymatic properties of the heterologously produced EGL1 are very
similar to those of the native enzyme. Grape maceration in the presence of
culture filtrate enriched in EGL1 resulted in increased release of aroma pr
ecursors, particularly in the case of aromatic grapes. Cryoscanning electro
n microscopy of the flesh of grapes treated with EGL1-enriched culture filt
rate revealed degradation of the cell wall matrix.