Liquid chromatography-UV irradiation-hydride generation-atomic fluorescence spectrometry for selenium speciation

The coupled technique liquid chromatography-UV irradiation-hydride generati on-atomic fluorescence spectrometry (LC-UV-HG-AFS) is proposed for selenite , selenate, selenocystine (SeCys) and selenomethionine (SeMet) determinatio n. Separation was performed by using an anionic exchange column and a phosp hate buffer as the mobile phase. Hydride generation and UV irradiation were optimised. The detection limits obtained permitted the determination of th e four species;at trace level concentrations. The method was validated by a nalysing two water certified reference materials, in which only selenite wa s detected.