In vitro maturation and fertilization of immature oocytes: A comparative study of fertilization techniques

Purpose: Our purpose was to investigate the factors influencing maturation and fertilization of immature oocytes. Methods: Immature oocytes were obtained from women undergoing cesarean sect ion. They were cultured in the maturation medium either with or without cum ulus cells. After maturation to metaphase II, they were randomly fertilized by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) . Results: After incubation for 48 hr 441 oocytes (42.8%) reached metaphase I I. Among them, 56.6% of cumulus-enclosed oocytes, but only 29.2% of denuded oocytes, reached metaphase II. Of the 289 cumulus-enclosed oocytes, the fe rtilization rates by IVF and ICSI were 56.3 and 84.1%, respectively (P < 0. 01). Of the 152 denuded oocytes, the fertilization rates by IVF and ICSI we re 39.5 and 84.5%, respectively (P < 0.01). The cleavage rates, however wer e similar. Conclusions: Cumulus cells are beneficial in the maturation of human oocyte s in vitro and that ICSI increases the fertilization rate for the in vitro matured oocytes. The developmental potential of the fertilized oocytes, how ever is similar irrespective of the fertilization method or the presence or absence of cumulus cells.