Development and robustness testing of a nonaqueous capillary electrophoresis method for the analysis of nonsteroidal anti-inflammatory drugs

Citation
S. Cherkaoui et Jl. Veuthey, Development and robustness testing of a nonaqueous capillary electrophoresis method for the analysis of nonsteroidal anti-inflammatory drugs, J CHROMAT A, 874(1), 2000, pp. 121-129
Citations number
28
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
Volume
874
Issue
1
Year of publication
2000
Pages
121 - 129
Database
ISI
SICI code
Abstract
Nine non steroidal anti-inflammatory drugs were simultaneously separated by nonaqueous capillary electrophoresis with a methanol-acetonitrile (40:60, v/v) mixture containing 20 mM ammonium acetate. The effect of solvent compo sition, electrolyte nature and concentration on the electrophoretic behavio r of the selected drugs was systematically studied. Investigated electrolyt es were ammonium, lithium and sodium acetate. Modification of the solvent a nd/or the electrolyte composition was found to alter the migration order of the pharmaceutical drugs. Finally, to assess method robustness, three sens itive electrophoretic parameters as well as their interactions were evaluat ed using a full factorial design at two levels. (C) 2000 Elsevier Science B .V. All rights reserved.