Kinetics of appearance of hemorphins from bovine hemoglobin peptic hydrolysates by a direct coupling of reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry

Reversed-phase high-performance liquid chromatography coupled with electros pray ionization mass spectrometry was used to improve the preparation of th ree opioid peptides (Leu-Val-Val-hemorphin-7, Val-Val-hemorphin-7 and Val-V al-hemorphin-4) resulting from bovine hemoglobin peptic hydrolysates, Optim al conditions for the preparation of these peptides were determined thanks to their kinetic studies of appearance in the course of peptic hydrolyses a s a function of degree of hydrolysis of hemoglobin. We propose a low degree of hydrolysis (3%) to prepare Leu-Val-Val-hemorpbin-7, a mean degree of hy drolysis (11%) to prepare Val-Val-hemorphin-7 and a high degree of hydrolys is (21%) to prepare Val-Val-hemorphin-4 (C) 2000 Elsevier Science B.V. All rights reserved.