Effects of DETANONOate, a nitric oxide donor, on hemostasis in rabbits: Anin vitro and in vivo thrombelastographic analysis

Purpose:The purpose of this study was to determine ii whole blood thrombela stographic variables (reaction time, K, alpha, and maximum amplitude) would be adversely effected by exposure to the nitric oxide (NO) donor, DETANONO ate, in vitro or after alveolar instillation in vivo. Materials and Methods: Conscious rabbits (n = 10) had blood sampled from ea r arteries anticoagulated with sodium citrate. The blood was then incubated with 0, 1, 5, 10, or 20 mmol/L DETANONOate for 30 minutes. Arterial blood from anesthetized rabbits (n = 4) was obtained and anticoagulated before an d 60 minutes after 1 mmol/L DETANONOate (2 mL/kg) was instilled into the ri ght lung. After incubation, all samples were placed in a thrombelastograph and recalcified, with thrombelastographic variables measured for 45 minutes . Results: In vitro, 10 mmol/L DETANONOate significantly (P < .05) increased reaction time, K, and decreased or compared with values observed after incu bation with 0, 1, and 5 mmol/L DETANONOate. Twenty mmol/L DETANONOate signi ficantly (P < .05) increased reaction time, K, and decreased or and maximum amplitude values compared with all other concentrations. In vivo, DFTANONO ate administration did not significantly affect thrombelastographic variabl es. Conclusion: DETANONOate significantly decreased hemostatic function in vitr o in a dose-dependent fashion but did not significantly affect hemostatic f unction in vivo. Copyright (C) 2000 by W.B. Saunders Company.