Vg. Nielsen et al., Effects of DETANONOate, a nitric oxide donor, on hemostasis in rabbits: Anin vitro and in vivo thrombelastographic analysis, J CRIT CARE, 15(1), 2000, pp. 30-35
Purpose:The purpose of this study was to determine ii whole blood thrombela
stographic variables (reaction time, K, alpha, and maximum amplitude) would
be adversely effected by exposure to the nitric oxide (NO) donor, DETANONO
ate, in vitro or after alveolar instillation in vivo.
Materials and Methods: Conscious rabbits (n = 10) had blood sampled from ea
r arteries anticoagulated with sodium citrate. The blood was then incubated
with 0, 1, 5, 10, or 20 mmol/L DETANONOate for 30 minutes. Arterial blood
from anesthetized rabbits (n = 4) was obtained and anticoagulated before an
d 60 minutes after 1 mmol/L DETANONOate (2 mL/kg) was instilled into the ri
ght lung. After incubation, all samples were placed in a thrombelastograph
and recalcified, with thrombelastographic variables measured for 45 minutes
.
Results: In vitro, 10 mmol/L DETANONOate significantly (P < .05) increased
reaction time, K, and decreased or compared with values observed after incu
bation with 0, 1, and 5 mmol/L DETANONOate. Twenty mmol/L DETANONOate signi
ficantly (P < .05) increased reaction time, K, and decreased or and maximum
amplitude values compared with all other concentrations. In vivo, DFTANONO
ate administration did not significantly affect thrombelastographic variabl
es.
Conclusion: DETANONOate significantly decreased hemostatic function in vitr
o in a dose-dependent fashion but did not significantly affect hemostatic f
unction in vivo. Copyright (C) 2000 by W.B. Saunders Company.