Utilization of microgravity to improve the crystal quality of biologicallyimportant proteins: chaperonin-60, GrpE, B-subunit of V-type ATPase, and MIF

Authors
Kitano, K Sasaki, R Nogi, T Fukami, TA Nakagawa, A Miki, K Tanaka, I
Citation
K. Kitano et al., Utilization of microgravity to improve the crystal quality of biologicallyimportant proteins: chaperonin-60, GrpE, B-subunit of V-type ATPase, and MIF, J CRYST GR, 210(4), 2000, pp. 819-823
Citations number
10
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF CRYSTAL GROWTH
ISSN journal
00220248 → ACNP
Volume
210
Issue
4
Year of publication
2000
Pages
819 - 823
Database
ISI
SICI code
0022-0248(200003)210:4<819:UOMTIT>2.0.ZU;2-4
Abstract
Protein crystallization experiments under microgravity were performed by us ing commercial vapor diffusion apparatus (CVDA) hardware on the STS-84 shut tle mission. Chaperonin-60, GrpE, the B subunit of V-type ATPase, and the s elenomethionine substituted proteins of macrophage migration inhibitory fac tor (MIF) were selected as target proteins. The results indicate that micro gravity positively affected their crystal growth, supporting the practical utilization of microgravity for the protein structure determination. (C) 20 00 Elsevier Science B.V. All rights reserved.