Cytokine-induced Src homology 2 protein (CIS) promotes T cell receptor-mediated proliferation and prolongs survival of activated T cells

Members of the suppressor of cytokine signaling (SOCS) family were discover ed as negative regulators of cytokine signaling by inhibition of the Janus kinase-signal transducer and activator of transcription (Jak-STAT) pathway. Among them, cytokine-induced Src homology 2 (SH2) protein (CIS) was found to inhibit the interleukin 3- and erythropietin-mediated STAT5 signaling pa thway. However, involvement of SOCS proteins in other signaling pathways is still unknown. This study shows that the expression of CIS is selectively induced in T cells after T cell, receptor (TCR) stimulation. In transgenic mice, with selective expression of CIS in CD4 T cells, elevated CIS strongl y promotes TCR-mediated proliferation and cytokine production in vitro, and superantigen-induced T cell activation in vivo. Forced expression of CIS a lso prolongs survival of CD4 T cells after TCR activation. Molecular events immediately downstream hom the TCR are not changed in CIS-expressing CD4 T cells, but activation of mitogen-activated protein (MAP) kinase pathways b y TCR stimulation is significantly enhanced. Together with the increased MA P kinase activation, a direct interaction of CIS and protein kinase CB was also demonstrated. These results suggest that CIS is one of the important r egulators of TCR-mediated T cell activation. The functions of CIS, enhancin g TCR signaling and inhibiting cytokine signaling, may be important in the regulation of immune response and homeostasis.