Cutting edge: Identification of the orphan receptor G-protein-coupled receptor 2 as CCR10, a specific receptor for the chemokine ESkine

A number of orphan G-protein coupled receptors (CPR) have been reported as putative chemokine receptors, One previously reported orphan receptor is an incomplete PCR clone, called GPR2. Here we report the cloning of full-leng th human (h)GPR2 and mouse (m)GPR2 cDNAs, and the identification of GPR2 as a receptor for a novel CC chemokine called ESkine, hGPR2 is expressed at h igh levels in testis and small intestine, and at lower levels in other tiss ues, mGPR2 was expressed at high le, els in small intestine, colon, lymph n odes, and Peyer's patches and at lower levels in thymus and spleen. Stimula tion of L1.2/hGPR2 transfectants with hESkine induced their migration and r esulted in intracellular calcium mobilization, These results provide eviden ce that GPR2 is a specific receptor for ESkine, We propose that GPR2 be ren amed as CCR10, The expression pattern of mGPR2/CCR10 suggests that it may p lay a role in the homing/trafficking of leukocytes within intestinal and ly mphoid environments.