Coulomb blockade is observed in semiconductor quantum dots fabricated by sc
anning probe lithography. We demonstrate that by combining top gates with i
n-plane gates, the lithographic shape of a dot can be transferred into the
electron gas with high accuracy. Furthermore, by applying voltages to the i
n-plane gates, the number of electrons on the dot can be changed by more th
an 70 without changing its shape. Transport measurements in high magnetic f
ields and with positive top gate voltages applied indicate that the potenti
al walls of the nanostructure can be made very steep.