Controlling the hydration of covalently immobilised penicillin G amidase in low-water medium: properties and use of Celite R-640

Authors
Basso, A De Martin, L Ebert, C Gardossi, L Linda, P
Citation
A. Basso et al., Controlling the hydration of covalently immobilised penicillin G amidase in low-water medium: properties and use of Celite R-640, J MOL CAT B, 8(4-6), 2000, pp. 245-253
Citations number
22
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN journal
13811177 → ACNP
Volume
8
Issue
4-6
Year of publication
2000
Pages
245 - 253
Database
ISI
SICI code
1381-1177(20000218)8:4-6<245:CTHOCI>2.0.ZU;2-U
Abstract
The study describes how Celite R-640 adsorbs liquid water in toluene and va pour water from a gas phase. In toluene, Celite R-640 is able to maintain t he water activity (a(w)) constant within broad ranges of water concentratio ns. The a(w) values are strongly related to the original hydration of the C elite batches, but prolonged drying confers comparable and reproducible pro perties to the different batches. The use of Celite R-640 in controlling th e hydration and activity of covalently immobilised PGA in toluene is report ed. (C) 2000 Elsevier Science B.V. All rights reserved.