The BFRF1 gene of Epstein-Barr virus encodes a novel protein

Computer analysis of the Epstein Barr virus (EBV) genome indicates there ar e similar to 100 open reading frames (ORFs). Thus far about 30 EBV genes di vided into the categories latent and lytic have been identified. The BamHI F region of EBV is abundantly transcribed during lytic replication. This re gion is highly conserved among herpesviruses, thus suggesting that some com mon function could be retained in the ORFs encompassed within this viral fr agment. To identify putative novel proteins and possible new markers for vi ral replication, we focused our attention on the first rightward ORF in the BamHI F region (BFRF1). Histidine and glutathione S-transferase-tagged BFR F1 fusion proteins were synthesized to produce a mouse monoclonal antibody (MAb). Analysis of human sera revealed a high seroprevalence of antibodies to BFRF1 in patients affected by nasopharyngeal carcinoma or Burkitt's lymp homa, whereas no humoral response to BFRF1 could be detected among healthy donors. An anti-BFRF1 MAb recognizes a doublet migrating at 37 to 38 kDa in cells extracts from EBV-infected cell lines following lytic cycle activati on and in an EBV-negative cell line (DG75) transfected with a plasmid expre ssing the BFRF1 gene. Northern blot analysis allowed the detection of a maj or transcript of 3.7 kb highly expressed in EBV-positive lytic cycle-induce d cell lines. Treatment with inhibitors of viral DNA polymerase, such as ph osphonoacetic acid and acyclovir, reduced but did not abolish the transcrip tion of BFRF1, thus indicating that BFRF1 can be classified as an early gen e. Cell fractionation experiments, as well as immunolocalization by immunof luorescence microscopy, immunohistochemistry, and immunoelectron microscopy , showed that BFRF1 is localized on the plasma membrane and nuclear compart ments of the cells and is a structural component of the viral particle. Ide ntification of BFRF1 provides a new marker with which to monitor EBV infect ion and might help us better understand the biology of the virus.