To test the effects of different sources of tremor on the voice, tremor was
simulated by external rhythmic perturbation of structures at the subglotta
l, glottal, and supraglottal levels in 10 healthy subjects. The acoustic an
d airflow signals simultaneously recorded during sustained phonation in the
normal and the 3 simulated tremor conditions were analyzed and compared. V
oice measures included: fundamental frequency, 2 short-term perturbation me
asures (jitter and shimmer), and 3 long-term tremor measures (prominence ra
tios of the spectral peaks of the acoustic frequency contour, acoustic ampl
itude contour, and airflow contour). Measures of fundamental frequency and
percent shimmer were not significantly affected by the simulated tremors. M
easures of percent jitter and the amplitudes of the long-term frequency and
amplitude modulations were most prominently increased when respiratory dri
ve was perturbed by simulated tremor. Spectral analysis of the acoustic amp
litude contour was most useful in distinguishing the 3 sites of simulated t
remor.