Triton X-114 phase partitioning for the isolation of a pediocin-like bacteriocin from Carnobacterium divergens

A new procedure combining Triton X-114 phase partitioning and cation exchan ge chromatography was developed to purify a bacteriocin from a complex cult ure medium. This pediocin-like bacteriocin, secreted by Carnobacterium dive rgens and named divercin V41, was entirely recovered in the lower detergent -rich phase whereas all other substances (compounds from culture medium, ba cterial metabolites) remained in the upper detergent-poor phase. Subsequent cation-exchange chromatography of the TX-114-rich phase allowed recovery o f the pure active bacteriocin and also detergent removing. This new purific ation method is versatile, fast (only two steps) and call be carried out on whole broth.