Immunohistochemical analysis of expression of a 65 kDa oncofetal protein (p65), epidermal growth factor receptor (EGFR), oncogene c-erb B2 and tumor suppressor gene p53 protein products in breast cancer patients

Paraffin-embedded tissue slides from 88 infiltrating ductal breast carcinom a were examined by immunohistochemistry technique with the use of monoclona l antibody against human p65 antigen and polyclonal antibody against p65-li ke protein present in fetal bovine serum. Immunohistochemical analysis of e xpression of growth Factor receptors (EGFR), protein product of oncogene c- erb B2 as well as protein product of mutated anti-oncogene p53 was also don e. It was established that there is no correlation between p65 and c-erbB2, EGFR or p53 expression. Tn low differentiated tumors (grade III) high p53 index and high EGFR and c-erbB2 expression was connected with low p65 expre ssion, The lack of c-erbB2 and EGFR and low p53 expression was combined usu ally with high p65 oncoproteine levels.