L. Hunakova et al., Radiation-induced apoptosis and cell cycle alterations in human carcinoma cell lines with different radiosensitivities, NEOPLASMA, 47(1), 2000, pp. 25-31
Radiosensitivity of examined human neoplastic cell lines was assessed with
the aid of MTT assay. Differences between radiosensitive and radioresistant
human neoplastic cell lines were as follow: a) radiation-induced apoptosis
detected by flow cytometry was apparent in the most radiosensitive (i.e. C
H-1 ovarian carcinoma cell line), but not in the radioresistant (i.e. SKOV-
3 ovarian carcinoma) cell lines, b) radiation-induced G(2)/M arrest appeare
d early after irradiation (6 hours) in both the radioresistant SKOV-3 cells
and in the radiosensitive CH-1 human ovarian carcinoma cell line, but a di
fferent pattern was observed 24 hours after irradiation with 2 Gy dose with
G(2)/M arrest only in radiosensitive cell line. The radiosensitivity and r
esistance to radiation-induced apoptosis in the radioresistant human breast
carcinoma MDA-MB-231 cell line were similar to those observed in SKOV-3 ce
lls. These data suggest that radiation-induced apoptosis and cell cycle alt
erations can predict radiosensitivity at least in some examined human malig
nant cells in vitro.