Identification of stable maintainer and fertility restorer lines for 'Polima' CMS in Brassica campestris

'Polima' cytoplasmic male sterility (CMS) was transferred from 'Polima' Bra ssica napus 'ISN 706' to five different cultivars of Brassica campestris (' Pusa kalyani', 'Pant toria', 'Candle', 'Tobin' and 'ATC 94211') by repeated backcrossing. It was observed that, while 'Polima' CMS manifested complete and stable male sterility in the nuclear backgrounds of 'Pusa kalyani', 'P ant toria', and 'Tobin', the cultivars 'Candle' and 'ATC 94211' possessed t he restorer gene for this CMS in the heterozygous condition. An analysis of F-1 and F-2 generations of 'Polima' 'Pusa kalyani' x 'Candle' and 'Polima' 'Pusa kalyani' x 'ATC 94211' revealed that restoration is controlled by a single dominant gene. Identification of stable maintainers and restorers of 'Polima' CMS could facilitate the development of hybrid varieties in B. ca mpestris.