Modulation of humoral and cell-mediated immune responses by dietary luteinin cats

The immuno-modulatory role of dietary lutein in domestic cats is unknown. F emale Tabby cats (10-month old, n=56) were supplemented daily for 12 weeks with 0, 1, 5 or 10 mg lutein. Blood was collected on Weeks 0, 2, 4, 8 and 1 2 to assess the following: (1) mitogen-induced peripheral blood mononuclear cells (PBMCs) proliferation, (2) changes in PBMC subpopulations, (3) inter leukin-2 (IL-2) production and (4) plasma immunoglobulin (Ig)G production, in addition, delayed-type hypersensitivity (DTH) response to concanavalin A (Con A) or a polyvalent vaccine was performed on Weeks 0, 6 and 12. Dietar y lutein increased plasma lutein concentrations in a dose-dependent manner (p<0.001) and concentrations had not reached steady state after 12 weeks of feeding in cats given 5 or 10 mg lutein. Concentrations of plasma retinol and a-tocopherol were not influenced by diet. The DTH response to vaccine b ut not to Con A increased (p<0.05) in a dose-dependent manner on Week 6. Co mpared to control, cats fed lutein also showed enhanced Con A- and pokeweed mitogen-stimulated PBMCs proliferation. Dietary lutein also increased the percentages of CD4+ and CD21+ lymphocytes on Week 12 but had no significant effect on pan T, CD8 and MHC class II markers. Plasma IgG was higher (p<0. 05) in cats fed 10 mg lutein on Weeks 8 and 12. These results support the i mmuno-modulatory action of lutein in domestic cats. (C) 2000 Published by E lsevier Science B.V. All rights reserved.