Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis

Authors
Osipiuk, J Sriram, M Mai, XH Adams, MWW Joachimiak, A
Citation
J. Osipiuk et al., Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis, ACT BIOCH P, 47(1), 2000, pp. 209-214
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ACTA BIOCHIMICA POLONICA
ISSN journal
0001527X → ACNP
Volume
47
Issue
1
Year of publication
2000
Pages
209 - 214
Database
ISI
SICI code
0001-527X(2000)47:1<209:CEACOC>2.0.ZU;2-K
Abstract
Two genes of the extreme thermophilic archaeon Thermococcus litoralis homol ogous to those that code for Cpn60 chaperonins were cloned and expressed in Escherichia coli. Each of the Cpn60 subunits as well as the entire Cpn60 c omplex crystallize in a variety of morphological forms. The best crystals d iffract to 3.6 Angstrom resolution at room temperature and belong to the sp ace group I422 with unit cell parameters a = b = 193.5 Angstrom, c = 204.2 Angstrom.