An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism
R. Fontes et al., An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism, ACT BIOCH P, 47(1), 2000, pp. 259-268
It is not always clear that some equations affected by complicated factors
can, actually, be interpreted as a ratio of two polynomials of first degree
and so that they can be, in general, represented by rectangular hyperbolas
. In this paper we present an easy procedure to rearrange those equations i
nto Michaelis-Menten-type equations and so to make the aspects of these rec
tangular hyperbolas more clear, particularly for researchers familiar with
general biochemistry. As an example, the method is applied to transform the
classical rate equation of the Cleland's Ordered Uni Bi enzyme mechanism.