An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism

Citation
R. Fontes et al., An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism, ACT BIOCH P, 47(1), 2000, pp. 259-268
Citations number
7
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ACTA BIOCHIMICA POLONICA
ISSN journal
0001527X → ACNP
Volume
47
Issue
1
Year of publication
2000
Pages
259 - 268
Database
ISI
SICI code
0001-527X(2000)47:1<259:AEPTTT>2.0.ZU;2-X
Abstract
It is not always clear that some equations affected by complicated factors can, actually, be interpreted as a ratio of two polynomials of first degree and so that they can be, in general, represented by rectangular hyperbolas . In this paper we present an easy procedure to rearrange those equations i nto Michaelis-Menten-type equations and so to make the aspects of these rec tangular hyperbolas more clear, particularly for researchers familiar with general biochemistry. As an example, the method is applied to transform the classical rate equation of the Cleland's Ordered Uni Bi enzyme mechanism.