T. Domon et al., Ultrastructural study of the root dentine surface resuming resorption on human deciduous teeth, ANN ANATOMY, 182(2), 2000, pp. 175-184
Resorption of deciduous teeth is not continuous, but alternates with period
s of repair or rest. Dentine surfaces in periods of rest or repair resume r
esorption by odontoclasts during physiological root resorption of the decid
uous teeth. However, no observations of such dentine surfaces have been sho
wn. The characteristic feature of the dentine surfaces resuming resorption
remains unknown. Tartrate-resistant acid phosphatase activity (TRAP) was de
tected on human deciduous teeth. The root resorbing surfaces on these teeth
were photographed with a whole-mount light microscope, and the photographe
d areas were serially sectioned into 0.5 mu m semithin sections. Preodontoc
lasts and odontoclasts were three-dimensionally reconstructed. On root reso
rbing surfaces, areas with small scattered TRAP-positive cells were observe
d among areas with many TRAP-positive resorbing odontoclasts and TRAP-negat
ive areas. The sections showed that areas with small scattered TRAP-positiv
e cells have features similar to those of TRAP-negative areas, but there we
re three kinds of characteristic TRAP-positive cells: preodontoclasts, odon
toclasts forming small lacunae, and preodontoclasts and odontoclasts with c
ytoplasmic processes extending to the dentine surface, which is covered wit
h cells. These results suggest that the areas with small scattered TRAP-pos
itive cells could be at the stage of resuming resorption, and show that the
presence of preodontoclasts and odontoclasts with cytoplasmic processes ex
tending to the covered dentine surface is a characteristic feature of the d
entine surface at this stage.