The effects of granulocyte colony stimulating factor on chemiluminescence and lipid peroxidation of blood platelets treated with cisplatin

The effects of granulocyte colony stimulation factor (G-CSF) at concentrati ons of 0.08, 0.8 and 8 mu g/ml on reactive oxygen species (ROS) generation and lipid peroxidation induced by cisplatin in pig blood platelets were inv estigated. The revel of reactive oxygen species (O-2(.-), H2O2, singlet oxy gen and organic radicals) generated in platelets was measured by the chemil uminescence method. Lipid peroxidation was determined by the thiobarbituric acid technique and was expressed as thiobarbituric acid reactive substance s. G-CSF at the concentration of 0.08 mu g/ml had a strong inhibitory effec t (about 60% inhibition) on the production of ROS in the isolated pig plate lets. This cytokine also significantly reduced lipid peroxidation in contro l platelets and platelets treated with cisplatin (p < 0.05). In the presenc e of G-CSF in the incubation medium (0.8 mu g/ml) cisplatin-induced generat ion of nos was also reduced (p < 0.05). This study demonstrates that G-CSF has a protective effect against the oxidative stress in blood platelets cau sed by cisplatin. [(C) 2000 Lippincott Williams & Wilkins.].