Determination of the maximal carcinoma/normal skin ratio after HpD or m-THPC administration in Hairless mice (SKH-1) by fluorescence spectroscopy

The two major steps in our study on the treatment of skin carcinomas by pho tochemotherapy (PCT) were the development of a skin tumor model in Hairless mice by a chemical carcinogenesis and the use of fluorescence spectroscopy , a semi-quantitative and non-invasive method, in order to determine the ti me after i.p. injection of photosensitizer when the tumor/normal skin ratio was the highest. A three-step carcinogenesis protocol provided mice bearin g carcinomas and these were used to determine the tumor/normal skin ratios of two photosensitizers by fluorescence spectroscopy, Hematoporphyrin deriv ative (HpD) (5 mg/kg body weight) and m-tetra(hydroxyphenyl) chlorine (m-TH PC) (0.3 mg/kg body weight) were injected i.p,, and fluorescence was measur ed at 1, 4, 8, 12, 24, 48, 72 and 96 h after injection. The best carcinoma/ normal skin ratio would be 3.2+/-1.4 for HPD and 2.7+/-2.1 for m-THPC, resp ectively. The delays required to reach these ratios were 72 h for HpD and 2 4 h for m-THPC. These results have to be considered with caution due to the high SEs and they must be confirmed by organic extraction, Photodynamic th erapy with the same doses of HpD and m-THPC used in this pharmacokinetic st udy has to be carried out in order to compare the toxicities of the two pho tosensitizers and to determine which one is the best for this type of tumor . [(C) 2000 Lippincott Williams & Wilkins.].