The construction of defective interfering rubella virus particles

Authors
Terry, G Londesborough, P Ho, L
Citation
G. Terry et al., The construction of defective interfering rubella virus particles, ARCH VIROL, 145(3), 2000, pp. 625-633
Citations number
11
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
03048608 → ACNP
Volume
145
Issue
3
Year of publication
2000
Pages
625 - 633
Database
ISI
SICI code
0304-8608(2000)145:3<625:TCODIR>2.0.ZU;2-Z
Abstract
Two defective viral vectors containing nucleic acid sequences from rubella virus strains RJ and RS linked to the reporter gene luciferase have been co nstructed. The vector RNAs are shown to replicate and interfere with wildty pe virus in co-infected cells. The interference rs associated with a polymo rphic nucleotide at nt34 in the 5' stem-loop. The use of these constructs a s expression vectors is discussed.