Bioheap leaching of secondary copper ores is applied commercially at operat
ions in Chile and Australia. Bioheap leaching of sulfidic refractory gold o
res has been demonstrated at large scale. There is limited comprehension of
what actually occurs microbiologically in full-scale bioheap operations, d
espite the commercial achievement of copper ore bioheap leaching and the an
ticipated technical and commercial success of gold ore bioheap leaching. Co
pper bioheaps are typically inoculated with the bacteria contained in the r
affinate, whereas, sulfidic refractory gold ore bioheaps can be inoculated
with bacteria developed in a separate reactor. Chemical and physical condit
ions within bioheaps change radically from the time the bioheap is stacked
and inoculated until bioleaching is completed. Redox, acidity, temperature,
oxygen and solution chemistry conditions can vary widely during the oxidat
ion period. Such conditions likely select for microorganisms or may, in fac
t, effect a succession of organisms in portions of the bioheap. Bioheap sol
utions are recycled and constituent build-up over time also affects the mic
robiology. Heterotrophic microorganisms may also play some role in bioheap
leaching. Understanding the microbiology of bioheaps is key to advancing co
mmercial bioheap applications. Such knowledge will increase the ore types a
s well as the diversity of mineral deposits that can be processed by biohea
p technology. It will also enable better control of conditions to improve l
each rates, metal recoveries and costs. This paper briefly explains commerc
ial practices, describes chemical, physical and microbiological monitoring
of bioheap, considers conditions that control microbial populations in bioh
eaps, and examines the types of ore deposits that could be bioleached, if t
he microbiology was elucidated.