Isolation and some properties of a serine protease from the fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam.

Authors
Uchikoba, T Arima, K Shimada, M Yonezawa, H Kaneda, M
Citation
T. Uchikoba et al., Isolation and some properties of a serine protease from the fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam., BIOS BIOT B, 64(3), 2000, pp. 623-624
Citations number
6
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
3
Year of publication
2000
Pages
623 - 624
Database
ISI
SICI code
0916-8451(200003)64:3<623:IASPOA>2.0.ZU;2-F
Abstract
An endopeptidase (Cudrania protease) with molecular mass of 76 kDa has been purified from the fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam . The enzyme was stable between pH 6 and 10 at 30 degrees C for 60 min. The enzyme activity was inhibited by diisopropyl fluorophosphate, chymostatin, and aprotinin, but not by EDTA or pepstatin. These results indicated that the enzyme was a serine protease.