A hyper-thermostable, alkaline lipase from Pseudomonas sp with the property of thermal activation

Authors
Rathi, P Bradoo, S Saxena, RK Gupta, R
Citation
P. Rathi et al., A hyper-thermostable, alkaline lipase from Pseudomonas sp with the property of thermal activation, BIOTECH LET, 22(6), 2000, pp. 495-498
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
6
Year of publication
2000
Pages
495 - 498
Database
ISI
SICI code
0141-5492(200003)22:6<495:AHALFP>2.0.ZU;2-I
Abstract
A hyper-thermostable, alkaline lipase from a newly-isolated, mesophilic Pse udomonas sp. was optimal at pH 11 and at 90 degrees C. It had a half-life o f more than 13 h at 90 degrees C. It was activated by 30% when heated at 90 degrees C for 2 h. The enzyme had a greater affinity for mustard oil (K-m = 40 mg ml(-1)) than for olive oil (K-m = 140 mg ml(-1)).