Stabilization and activity-enhancement of mandelate racemase from Pseudomonas putida ATCC 12336 by immobilization

Authors
Strauss, UT Kandelbauer, A Faber, K
Citation
Ut. Strauss et al., Stabilization and activity-enhancement of mandelate racemase from Pseudomonas putida ATCC 12336 by immobilization, BIOTECH LET, 22(6), 2000, pp. 515-520
Citations number
19
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
6
Year of publication
2000
Pages
515 - 520
Database
ISI
SICI code
0141-5492(200003)22:6<515:SAAOMR>2.0.ZU;2-M
Abstract
Mandelate racemase [EC 5.1.2.2] from Pseudomonas putida ATCC 12336 was effi ciently immobilized through ionic binding onto DEAE- and TEAE 23-cellulose. The activity of the immobilized enzyme was significantly enhanced as compa red to the native protein, i.e., 2.7- and 2.5-fold, respectively. DEAE-cell ulose-immobilized mandelate racemase could be efficiently used in repeated batch reactions for the racemization of (R)-mandelic acid under mild condit ions.