Ai. Chapoval et al., In vitro growth inhibition of a broad spectrum of tumor cell lines by activated human dendritic cells, BLOOD, 95(7), 2000, pp. 2346-2351
Dendritic cells (DCs) are critical subsets of leukocytes providing antigen
presentation for initiation of humoral and cellular immune responses. Their
role as effector cells in tumor resistance, however, is less known. We rep
ort here that human DCs generated by culturing plastic-adherent peripheral
blood monocytes in the presence of granulocyte-monocyte colony-stimulating
factor (GM-CSF) and interleukin-4 have potent growth-inhibition activity in
vitro on a wide spectrum of human tumor lines of different tissue origin.
Proinflammatory stimuli lipopolysaccharide (LPS) and interferon-gamma, but
not tumor necrosis factor-alpha and CD40 signaling, can further enhance DC-
mediated inhibition of tumor growth. The growth inhibition requires contact
between nos and tumor cells while LPS treatment enhances the antitumor act
ivity in DC culture supernatants. Our results suggest that in addition to t
heir predominant role as regulatory cells, activated DCs are also potential
effector cells in tumor Immunity. (Blood, 2000;95: 2346-2351) (C) 2000 by
The American Society of Hematology.